Technical Description
The present invention relates to the HMG CoA reductase gene encapsulated inorganic metallic nanoparticle and its application in plant biotransformation. More particularly, invention relates to calcium phosphate nanoparticle preparation actively conjugated/encapsulated with HMG Co-A reductase genes and its application in plant biotransformation process. The invention further relates to a process of entrapping genetic material in nanoparticles of calcium phosphate of size below100nm diameter to form non-viral carrier which can be used suitably for the delivery of genes in the plant of Cichorium intybus L. The invention provides for the use of micellar solubilisation technique as a method of synthesis of HMG CoA reductase gene encapsulated calcium phosphate nanoparticle. The proposed method of biotransformation process in plant Cichorium intybus L. mediated by calcium phosphate nanoparticle encapsulated with HMG CoA reductase gene results in the biosynthesis enhancement of secondary plant metabolite.
Tech Features
The invention provides an improved method of gene transfer in plants that increases the gene transformation rate up to 30% and survival of transformed C. intybusupto50%
Target Audience
- Plant Biotechnology
