Technical Description
The technology discloses a fermentation process for producing high molecular weight hyaluronan (HA) using recombinant Lactococcus lactis MKG6. Acetate co-utilization and controlled feeding strategies adjust metabolic fluxes and acetyl-CoA availability. Anaerobic fermentation is reported to produce HA with a molecular weight of 3.4 MDa.
Problems Addressed
- Low HA Molecular Weight
- Unmet High-Molecular-Weight Demand
- Overlooked Cofactor Requirements
- Limited Acetate Co-utilization
- Insufficient Precursor Availability
- Biomedical Product Stability Needs
Tech Features
- Recombinant Lactococcus lactis MKG6
- HA Molecular Weight 3.4 MDa
- Acetate Co-utilization Strategy
- Acetyl-CoA and Metabolic Flux Management
- Acetate and Glucose Pulse Feeding
- pH Feedback
Target Audience
- Pharmaceutical Manufacturers
- Biomedical Product Developers
- Medical-Grade HA Producers
- Bioprocess Development Teams
Tech ID: P06-2312 TRL 4 Patent Status: Granted Available For Exclusive and Non-exclusive License
